Single-molecule labeling & localization microscopy (SMLLM)

Focus 1

THE PROBLEM: Superresolution imaging is revolutionizing biomedical research beyond the diffraction limit of visible light, first established by Ernst Abbe in 1873. Despite its enormous potential, technical challenges emerge as resolution increases. Artifacts associated with immunofluorescent labeling, error-prone molecular quantification, and the system’s high cost have hindered superresolution’s full potential.

OUR APPROACH: We take an interdisciplinary approach to advance superresolution techniques toward quantitative and multimodal imaging. Main methods include single-molecule and quantitative superresolution microscopy, immunofluorescence, molecular dynamics modeling, and antibody chemistry.

  • H Gunasekara⫽, T Perera⫽, J Anderson#, B Saed, N Ramseier, K Neama#, YS Hu*. Superresolution Imaging with Single-Antibody Labeling. Bioconjug. Chem. 2023;34(5):825-833. PMID: 37145839.. pdf Link
  • H Gunasekara, R Munaweera, L Novotná, BF Lillemeier*, YS Hu*, Chaotropic perturbation of noncovalent interactions of the hemagglutinin tag monoclonal antibody fragment enables superresolution molecular census, ACS Nano, 2022. PMID: 34797055 pdf Link